Effects of inhaling high concentrations hydrogen gas on neuronal apoptosis and the expression of apoptosis related proteins in the cortical region of rats with cerebral ischemia-reperfusion injury
XIA Yu-ning
LI Xue-mei
YUAN Nan-nan
ZHANG Xin-lei
TAN Yong-xing
Abstract：Objective To investigate the effects of inhalation of high concentrations hydrogen gas on neuronal ap -optosis and the expressions of apoptosis -related proteins Bcl -2 and Bax on focal cerebral ischemia reperfusion injury in rats.Methods Forty-eight healthy male SD rats were randomly divided into 3 groups (n=16), sham-operated group (sham group), ischemia-reperfusion group (I/R group, during reperfusion inhaled 67%N2 +33%O2) and inhalation of hydrogen group (H2 group, during reperfusion inhaled 67%H2 +33%O2).Middle cerebral artery occlusion (MCAO) model was constructed using conventional method .Neurological severity score was evaluated 24 h after cerebral reperfusion in all groups .The cerebral infarction severity and volume were assessed by TTC staining , and neuronal apoptosis of brain cortex was examined by Nissl stain and TUNEL technique .The expressions of Bcl -2 and Bax were assessed with Western blot.Results As compared with sham group , the neurological deficit score , brain infarction volume , apoptosis index (AI) and the expression of Bax in I/R group and H2 group were significantly increased (P<0.05).Compared with I/R group, the neurological deficit score , brain infarction volume , apoptosis index and the expression of Bax in H 2 group were markedly decreased , while the expression of Bcl -2 was dramatically increased , and so was the quantity of Nissel′s body (P<0.05).Conclusion High concentration hydrogen during reperfusion can protect the I /R rats by reducing the size of cerebral infarction , ameliorating the neurological deficit and neuron apoptosis , and which may be related with up -regula-ting Bcl-2 and down-regulating Bax .
Keywords：hydrogencerebral ischemia-reperfusionapoptosisapoptosis-related protein
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 3645-3649 )