A Comparative Analysis of Methylene Tetrahydrofolate Reductase Gene Detection Results between Foshan and Nationwide Results

HUANG Wu; QIU Xiao-min; WAN Xiao-liang; LIN Shao-hua; GAN Shu-guang; CHEN Mei-yu

doi:10.3969/j.issn.1001-9448.2016.23.014

Abstract：Objective To investigate the Methylene tetrahydrofolate reductase ( MTHFR ) gene distribution a-mong hypertensive patients in Foshan and compare with nationwide results .Methods MTHFR gene detection was per-formed in 998 cardiovascular outpatients and hypertensive inpatients in the Second People ′s Hospital of Foshan from April 2013 to September 2014.The polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method was applied for accurate gene mapping .Plasma HCY was determined by high -performance liquid chromatography with fluorescence detection (HPLC-FLD).Results Among 998 hypertensive patients (494 males and 504 females) selected for MTHFR gene detection , 82 patients were detected to be TT ( accounting for 8.22%) , while 338 patients were identi-fied as CT (accounting for 33.87%) and 578 patients as CC (accounting for 57.92%).Among them, there were 54 TT (10.93%) and 168 CT (34.01%) in males, as well as 28 TT (5.56%) and 170 CT (33.73%) in females.For TT type, the proportion of males was significantly higher than that of females (0.1093 vs.0.0556, P<0.01).Meanwhile, 739 patients were chosen for HCY detection (351 males and 388 females), and there were 389 H type hypertensive pa-tients, accounting for 52.64%, among whom there were 224 males (63.82%) and 165 females (42.53%).The propor-tion of males was significantly higher than that of females .Conclusion The TT gene type frequency in South China ( Fos-han) (8.22%) is lower than 25%, which is the nationwide frequency .The H type hypertension frequency in South China (52.64%) is also lower than 75%, which is the nationwide frequency .It is shown that there are regional differences in the distribution of gene polymorphism .The frequency of H type hypertension and TT type gene in males are higher than those in females .