Effects of electroacupuncture preconditioning on blood spinal cord barrier after spinal cord ischemia reperfusion injury
QIN Mei-man
LIU Ping
LI Yun
FANG Bo
Abstract：Objective To study the effects of electroacupuncture preconditioning on the blood spinal cord barrier after spinal cord ischemia reperfusion injury in rats .Methods Ninety SD rats were randomly divided into three groups:sham operation group (Group S), spinal cord ischemia reperfusion injury group (Group IR), and electroacupuncture pre-conditioning group ( Group E) .The ischemia reperfusion injury model was established in rats in Group IR and Group E u-sing thoracic aorta clamping .Rats in Group S were only experienced thoracic aorta exposure .Rats in Group E were treated with electroacupuncture pretreatment before injury .Motor function scores were recorded 48h after injury.The permeability of blood spinal cord barrier was determined by Evans blue fluorescence .Western blot was used to detect the expression of AQP-4 in the spinal cord tissues .Results Compared with the Group S , the neurological scores in Group IR were signifi-cantly reduced , with the permeability of blood spinal cord barrier significantly increased , and so was the expression of AQP-4 (P<0.05).Compared with Group IR, the neurological scores in Group E were increased , with the permeability of blood spinal cord decreased , and so did the expression of AQP -4 (P<0.05).Conclusion Electroacupuncture pre-conditioning can inhibit the expression of AQP -4 after spinal cord ischemia reperfusion injury , which reduce the break-down of blood spinal cord barrier permeability , and thus ameliorate the spinal cord ischemia reperfusion injury .
Keywords：Spinal cord ischemia reperfusion injuryElectroacupunctureAQP-4Blood spinal cord barrier
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 3494-3496 )