Effects of tetramethylpyrazine on human retinoblastoma cell line WERI-Rb1 and its mechanism
XU Li-jun
WU Nan-dan
YANG Ying
CHEN Pei
YU Ke-ming
ZHUANG Jing
Abstract：Objective To characterize the bioactivity of tetramethylpyrazine ( TMP) on human WERI -Rb1 cells and its transcription regulatory mechanism of TMP -mediated down-regulation of CXCR4.Methods (1) WERI-Rb1 cells were treated with TMP and AMD3100.The control cells were treated with DMSO or PBS at the same concentration . The morphology and cell density of WERI -Rb1 cells was observed and recorded by the living cells imager .(2)WERI-Rb1 cells were treated with TMP;then real-time RT-PCR, immunofluorescence and western blot were performed to de-tect the expression levels of CXCR4 and NRF1 in WERI-Rb1 cells treated with TMP.Results (1)TMP and AMD3100 notably inhibited the proliferation of WERI -Rb1 cells, as evidenced by cell morphology and density analysis .(2)TMP down-regulated CXCR4 and NRF1 expression in WERI-Rb1 cells at both mRNA and protein levels (P<0.05), as ev-idenced by real-time RT-PCR, immunofluorescence and western blot assays .Conclusion TMP significantly inhibits the proliferation of WERI -Rb1 cells and down-regulates its expression of CXCR 4 and NRF1.
Keywords：tetramethylpyrazineWERI-Rb1chemokine receptor 4nuclear respiratory factor 1
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2721-2724 )