some 38-degree liquor from Guizhou on the serum testosterone levels and the expression of StAR, EGF and CgA in the rat Leydig cells
LIU Hao
LI Yi-xin
YIN Dan
LI Rong-rong
XIA Bai-juan
WANG Yan-lin
CHEN Xing-qiang
LIANG Wen-mei
Abstract：Objective To investigate a brand of 38-degrees of edible alcohol from Guizhou on rat Leydig cells in steroidogenic acute regulatory protein (StAR), epidermal growth factor (EGF), chromogranin A (CgA) expression and serum testosterone.Methods Sixty normal adult male SD rats were randomly divided into normal control group (n=15) and experimental groups ( n =54 ) .Experimental groups included low dose group , mediate dose group and high dose group.The rats in experimental groups were given with corresponding dose of liquor gavage twice a day .At the end of the 4th, 8th and 12th week, the serums were collected and testicular tissue was taken .Immunohistochemical SABC method , image analysis, Western-blotting, chemiluminescence method were applied .Results There was no significant difference in serum T among all 4 groups ( P>0.05) .Compared with the normal control group , the expression of StAR was signifi-cantly up-regulated in all 3 experimental groups ( P<0.05 ) .Conclusion No serum testosterone alteration is observed in experiment groups , nor is the expression of EGF or CgA .StAR expression is enhanced by liquor intake .
Keywords：liquortestosteronesteroidogenic acute regulatory proteinepidermal growth factorchromogranin Achemiluminescence methodimmunohistochemicalWestern-blotting
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 2549-2554 )