Effect of ursolic acid on AP-1 and NF-κB expression in hepatic stellate cells

CHEN Tao; HE Wen-hua; HUANG Wen; YU Shan-shan; CHEN Biao; HUANG De-qiang; LI Bi-min; ZHU Xuan

Abstract：Objective To clarify the effects of NOX on the generation of ROS and the activity of AP -1 and NF-κB in rat hepatic stellate cells (HSC-T6) induced by AngⅡ.Methods Culture-activated HSC-T6 cells were divided as followed: AngⅡ group ( received AngⅡ); normal control group ( received no treatment ); and intervention groups, in which HSC-T6 cells were pretreated with UA (50 μmol/L) and NOX inhibitor DPI (20 μmol/L) for 30 min, and subsequently stimulated with AngⅡ for different time periods .The fluorescence intensity was examined with ROS detection kit and fluorescence microplate .The activities of AP-1 and NF-κB were determined using electrophoretic mobility shift assay (EMSA).Results After treated with AngⅡ for 30 min, the fluorescence intensity was higher than control group (P<0.05).After intervened by DPI and UA, the fluorescence intensity was significantly lower than that in AngⅡtreated groups (P<0.05).After treated with AngⅡfor one hour, the activities of AP-1 and NF-κB were sig-nificantly higher than control group (P<0.05).After intervened by DPI and UA, The activities of AP-1 and NF-κB were significantly lower than that in AngⅡtreated groups ( P<0.05 ) .Conclusion NOX can mediate the activation of AP-1 and NF-κB by influencing the generation of ROS , UA can reduce the activity of AP -1 and NF-κB by inhibi-ting generation of ROS .