The effect of IL-32γon CollagenⅠ expression by LX-2 cells

PAN Xing-fei; ZHANG Shao-quan; YANG Xiao-an; ZOU Xiao-fang; WEI Li-ping

Abstract：Objective To investigate whether could IL-32γinduce Collagen Ⅰ expression in LX-2 cells. Methods LX-2 cells were co-cultured with IL-32γof different concentrations.Then total RNA was extracted, and the cell supernatant was collected.CollagenⅠRNA expression level was assayed by using real-time PCR.CollagenⅠprotein expression level was detected by using ELISA.Subsequently, LX-2 cells were co-cultured with recombinant Human Active p38αprotein of different concentrations.Forty-eight hours later, the cell supernatant was collected and CollagenⅠprotein expression level was assessed.The LX-2 cells were then co-cultured with IL-32γ, and p38MAPK inhibitors SB 203580 of different concentrations were added.CollagenⅠRNA and protein expression levels were assessed by using real-time PCR and ELISA, respectively.Results IL-32γcould induce Collagen Ⅰ expression in LX-2 cells.Moreover, CollagenⅠexpression was induced by IL-32γand recombinant Human Active p38αprotein at dose-dependent manners.CollagenⅠexpression induced by IL-32γwas inhibited by p38MAPK.Conclusion IL-32γin-duces CollagenⅠexpression in LX-2 cells via the activation of p38MAPK signal pathway.IL-32 might be involved in the pathogenesis of liver fibrosis via the induction of CollagenⅠexpression in LX-2 cells.