Expression of hsa-miR-3658 and LASS2 genes in bladder cancer of different pathological grades and clinical stages

LIANG Min; WANG Hai-feng; WANG Jian-song; ZOU Ren-chao; YANG Hong

Abstract：Objective To investigate the expression of hsa -microRNA-3658 ( miR-3658) and Homo sapiens longevity assurance homologue 2 ( LASS2 ) genes in bladder cancer tissues with different pathological grades and clinical stages.Methods The expression of miR -3658 and LASS2 mRNA in 96 paired tissues, which acquired from bladder cancer and normal epithelium tissues beside the carcinoma , was assessed using quantitative reverse transcription PCR .The technology of immunohistochemistry stain ( IHC) was also performed to detect the expression of LASS 2 in these tissues . Results According to 2 -ΔΔCt Method, miR-3658 expression was significantly higher in bladder cancer tissues than adja-cent tissues, as the LASS2 expression was significantly lower in bladder cancer tissues (P<0.01).Both the expression of miR-3658 and LASS2 genes were different in bladder cancer tissues with different pathological grades and clinical stages . Compared to the highly differentiated bladder cancer tissues , poorly differentiated tissues presented with significantly up -regulated miR-3658 accompanied and down -regulated LASS2 gene ( P<0.01) .With increasing infiltration depth and clinical stages, the expression of miR-3658 was gradually increased, with reduction in the expression of LASS2 gene.At protein level, the LASS2 positive rate was significantly lower in bladder cancer tissues than adjacent tissues (P<0.01);as significantly lower LASS2 expression was observed in higher pathological grade , infiltration depth and clinical stage car-cinoma tissues (P<0.05).Conclusion LASS2 and miR-3658 expression may be involved in the development and progression of bladder cancer , and may be prognostic indicators for this cancer .