Impact of lentivirus -mediated gene silencing of growth factor independence -1 in chronic myeloid leukemia mononuclear cells
YANG Xi
LIU Hong
LIN Zeng-hua
LU Wei
ZHANG Ya-ping
Abstract：Objective To investigate effects of lentivirus-mediated gene silencing of growth factor independence-1 (GFI-1) on the growth and apoptosis of mononuclear cells in chronic myelogenous leukemia ( CML) patients, thus to e-valuate the significance of GFI -1 in pathogenesis of CML .Methods Peripheral blood and bone marrow samples were collected from 16 CML patients .As control group , another 17 samples were collected from normal healthy people .The ex-pression of GFI-1 in mononuclear cells in CML patients was silenced by using shRNA .After that the proliferative ability of mononuclear cells were tested by MTS assay , as apoptosis was examined by AnnexinV -propidium iodide(PI) staining, and DNA content was measuredby PI staining .Results GFI-1 expression was up-regulated in CML patients and sig-nificantly higher than that in control group ( P<0.001) .GFI-1 gene silencing can significantly inhibit mononuclear cells proliferation in 72 h (P<0.001) and induce apoptosis in mononuclear cells (P<0.005), which caused the retardation of cell cycle in mononuclear cells .Conclusion GFI-1 may contribute to inhibit cell proliferation by leading to apoptosis and retardation of cell cycle as a proliferation regulator .
Keywords：chronic myeloidleukemiagrowth factor independence -1mononuclear cellsproliferationapopto-siscell cycleRNA interference
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1944-1947 )