Construction and identification of eukaryotic expression plasmids pcDNA-FAPαand its variants
ZHAO Meng
SHAO Ting-ru
LV Xiao-zhi
Abstract：Objective To construct and identifythe eukaryotic expression plasmids containing wild -type FAPαand its mutants .Methods The wFAPαsequence and tFAPαsequence obtained by RT -PCR amplification using a high fidelity gene expression profiling strategy mediated by Pfu proofreading polymerases , were cloned into the eukaryotic ex-pression vector pcDNA3.1(+).The mFAPαwas obtained by overlap PCR using the pcDNA -wFAPαas template and cloned into the same vector .Results The construction of pcDNA-wFAPα, pcDNA-tFAPαand pcDNA-mFAPαwas confirmed by enzyme digestion and DNA sequencing .Conclusion The eukaryotic expression plasmids have been con-structed successfully , which lays important foundation for the future research on the molecular mechanism of FAP αin oral squamous cell carcinoma carcinogenesis .
Keywords：fibroblast activation protein αeukaryotic expression plasmidspcDNA3.1(+)
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1909-1911 )