Values of magnetic nanoparticles separation-based real-time fluorescent RT-PCR assay for detection of serum ;miR-21 in patients with esophageal squamous cell carcinoma
GUO Wen-tao
CHANG Ling-li
ZHAO Qing
ZHANG Qing-wei
ZHU Li-wei
LI Ya-guang
SUN Ning
ZHANG Wei
Abstract：Objective To establish the magnetic nanoparticles separation ( MS) -based real-time fluorescent RT-PCR assay for detection of serum micR-21 in patients with esophageal squamous cell carcinoma ( ESCC) , and to reveal the expression of miR-21 in serum of patients with ESCC. Methods Sixty cases of ESCC and 60 cases of healthy individuals were collected, and total serum RNA was extracted by Trizol and MS, respectively. The expression of miR-21 was detected by real-time fluorescent RT-PCR. The consistency of miR-21 expression between Trizol and MS ex-traction was detected by Bland-Altman method. Utility of miR-21 expression detected by MS in screening ESCC was e-valuated by ROC curves. Results The MS-based real-time RT-PCR was effective for separating and extracting serum miR-21, with sensitivity similar to the Trizol-based real-time RT-PCR. Bland-Altman results showed that the ex-pression of miR-21 extraced by the two methods had good consistency. The expression of miR-21 in the serum of ESCC patients was significantly higher than that in healthy individuals (P<0. 05). The area under the ROC curve was 0. 878. 1. 115 was the best critical value, where the sensitivity and specificity were 85. 00% and 81. 67%, respectively. The ex-pression of miR-21 in ESCC patients was correlated with TNM staging and lymph node metastasis. Conclusion MS-based real-time fluorescent RT-PCR assay is of great clinical significance in detection of serum miRNA. The expression level of miR-21 in serum should be a new biomarker for the diagnosis of esophageal carcinoma.
Keywords：magnetic nanoparticlesreal-time fluorescent PCRmiR-21esophageal squamous cell carcinoma
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 837-840 )