Establishment of Rat Model of the Qi-deficiency and diabetic nephropathy and its influential factors

FAN Shao-yi; ZHANG Xiu

Abstract：Objective To explore the method of establishing the rat model of Qi -deficiency and diabetic ne-phropathy and its influential factors .Methods 43 SPF male rats were divided randomly into 2 groups, the normal group (7 rats) and the model group (36 rats).The normal group was treated with normal diets .The model group were fed with high-fat diet followed by injection of STZ and were further divided randomly into the DN model group and DN with Qi -deficiency group .Rats in the DN with Qi-deficiency group were continuously fed with high -fat diet and underwent fa-tigue swimming test.Weight, urine volume, blood glucose, urinary microalbumine and pathologic changes in the kidney were monitored .Results The successful rate of STZ injection in establishment of the rat model of diabetic nephropathy was 83.3%, with 77.8% for single large -dose injection and 88.9% for multiple injections .The successful rate and mortality of DN with Qi -deficiency group were 60% and 28.6%, respectively .With increased duration of treatment , weight increased and 24 h urinary microalbumine decreased , while urinary volume , blood glucose and 24 h urinary protein decreased after initial rising .Conclusion The successful rate of the rat model of Qi -deficiency and diabetic nephropa-thy is high, but affected by many factors .