Changes of intestinal flora and effects of corresponding intervention in colon slow transit constipation rats

XIE Shang-kui; REN Dong-lin; XIAN Zheng-yu; Peng-hui; WANG Xiao-xue; LIN Hong-cheng; HU Bang

Abstract：Objective To explore changes of intestinal flora and effects of corresponding intervention in colon slow transit constipation ( STC) .Methods STC model was established by gastric infusion with compound diphenoxylate. Thirty-two STC rats were randomly divided into the STC group(n=16) and the intervention group(n=16).Sixteen nor-mal Spragne-Dawley rats were assigned to the control group(n=16).Intestinal flora were detected by 16SrRNA real-time PCR.Gut emptying time was measured through gastric infusion with activated carbon gastric infusion.Fecal bacteria in the STC group were detected before intervention based on the test results of intestinal flora.Fecal bacteria and gut emp-tying time were observed after the intervention.Results Fecal lactobacillus, bifidobacterium counts in the STC and con-trol groups were 4.22 ±0.07, 5.26 ±0.10 and 6.51 ±0.10, 7.68 ±0.17.These bacteria counts were lower in the STC group compared with those in the control group ( P<0.01 ) .Counts of total fecal bacteria, firmicutes, bacteroides and clostridium groupⅣin two groups was not statistically significantly different ( P>0.05 ) .After intervention with lactoba-cillusand bifidobacterium, counts of total fecal bacteria, firmicutes, bacteroides, clostridium groupⅣin STC, STC inter-vention and control group were not statistically significantly different (P>0.05).Lactobacillus and bifidobacterium counts in STC, STC intervention and the control groups were 4.20 ±0.07, 5.21 ±0.10 and 6.47 ±0.09, 6.47 ±0.05 and 6.50 ± 0.08, 7.79 ±0.06, respectively.Counts in the STC group were significantly lower than in the intervention group and the control group ( P<0.01 ) .Gut emptying time in the STC, STC intervention and control groups after intervention were (5.56 ±0.50) d, (2.55 ±0.67) d, (0.77 ±0.01) d.Gut emptying time in STC group was longer than the other two groups (P<0.01).Conclusions Intestinal lactobacillus and bifidobacterium counts in STC rats are reduced.There is no change in main functional bacteria in STC rats.Intervention with lactobacillus and bifidobacterium can restore intestinal microflora and gut emptying times.