Dynamic changes in early biomarkers of Caulis Aristolochiae Manshuriensis induced acute kidney injury in rats
ZHAO Jin
HU Qiu-yan
LI Si-ming
WU Zhi-feng
ZENG Xing
Abstract：Objective To investigate the changes of early biomarkers, such as neutrophil gelatinase-associated lipocalin( NGAL) , Cystatin C in blood and urine, as well as changes of total protein( TP) , albumin( ALB) in urine, in a rat model of acute kidney injury induced by Caulis Aristolochiae Manshuriensis(CAM).Methods Twenty-four male SD rats were randomly divided into 3 groups:the control group, the aristolochic acid-Ⅰ ( AAⅠ) group and the CAM group.Rats in the 3 groups received treatment with water, AAⅠat 36 mg/kg or CAM at 60 g/kg through gastric infusion for 3 days followed by a two-day break.Venous blood samples and urine samples were collected 1 day before treatments, 6 h and 12 h after each treatment.Blood, 24 h urine and kidney tissues were collected on the 5th day.At different time points, SCr, BUN, TP, GGT and ALB in urine or serum were measured.Secretion of NGAL and Cystatin C in urine or serum were determined by ELISA.Samples of kidney tissue were observed after HE staining.Results In the AAⅠ and CAM groups, urinary NGAL, TP, ALB and Cystatin C showed a significant increase and an upward trend with time ( P<0.05).Conclusion NGAL, TP, ALB and Cystatin C may be used as biomarkers for early diagnosis of kidney injury in-duced by CAM.
Keywords：Aristolochiae Manshuriensisearly kidney injuryCystatin Cneutrophil gelatinase -associated lipocalintotal proteinalbumin
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 321-324 )