Screening of differentially expressed genes related to artery calcification in rats by gene chip technology
LU Hai-lin
WEI Xiao-min
JIANG Wen-hong
HU Ming
YANG Han
QIN Xiao
Abstract：Objective To investigate the possible mechanism of arterial calcification by screening differentially expressed genes and detecting the expression levels of transforming growth factor beta ( TGF-β) , matrix metalloprotein-ase-2 ( MMP-2 ) and matrix metalloproteinase-9 ( MMP-9 ) between normal rats and rats with artery calcification through gene chip technology.Methods Arterial calcification in rats was induced by subcutaneous injection of large dose of vitamin D3 .The extent of artery calcification was determined by Von Kossa staining.Differentially expressed genes and the expression levels of TGF-β, MMP-2 and MMP-9 between normal rats and rats with arterial calcification were de-tected through gene chip technology.Results Compared with the control group, 710 genes were differentially expressed in the model group.344 were up-regulated and 366 were down-regulated.Expression levels of TGF-β1, TGF-β3, and MMP-2 in the model group were significantly up-regulated ( P<0.05) , while TGF-β2 and MMP-9 expression levels showed no significant difference between the two groups (P>0.05).Conclusion Multiple gene interaction con-tributes to the formation of artery calcification.Among them, TGF-β, MMP-2 and MMP-9 play a pivotal role in the pathogenesis of artery calcification.However, the mechanism still remains to be further explored.
Keywords：gene chiparterial calcificationtransforming growth factorβmatrix metalloproteinases
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 178-182 )