Effect of anti -c -myc small interference RNA on adrenocortical carcinoma cell proliferation
HUANG Xue-mei
LU De-cheng
LUO Zuo-jie
CHEN Bing
QIN Ying-fen
LIANG Xing-huan
ZHOU Jia
Abstract：Objective To investigate the effect of siRNA silencing of c-myc gene on cell proliferation in adreno-cortical carcinoma cell line SW-13 by siRNA interference of c-myc.Methods siRNA targeting c-myc gene was transfected into SW-13 cells.The c-myc mRNA and protein expression levels were detected by real-time quantitative PCR and Western blotting, respectively.MTT assay was used to assess cell proliferation.Expression of FHIT after trans-fection was assessed by real-time quantitative PCR.Results Expression of c-myc in adrenocortical carcinoma cells at mRNA and protein levels were suppressed 48 hours and 72 hours after transfection, respectively.The growth rate of adre-nocortical carcinoma cells SW-13 began to decline at 24 h after transfection, and was markedly suppressed at 48 and 72 hours after transfection.Expression of FHIT was increased when the c-myc expression was suppressed by siRNA.Con-clusion Silencing of the c-myc oncogene by siRNA could effectively inhibit proliferation of SW-13 cells.It provides the theoretical basis for targeted therapy of adrenocortical carcinoma.
Keywords：adrenocortical carcinomaC-mycFHITsiRNAtransfection
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 169-172 )