The protective effects of salvianolic acid B against doxorubicin-induced cardiotoxicity in H9c2 cells and the pre-diction of its targets
ZHUO Xian-jin
SHEN Hui-fang
FANG Zhi-chao
DING Yun-gang
WANG Tao
CUI Guo-zhen
Abstract：Objective To investigate the protective effects of salvianolic acid B ( Sal B ) against doxorubicin (Dox)-induced cardiac injury in H9c2 cells and its possible targets.Methods H9c2 cells treated with Dox and Sal B were used to evaluate cell protective effects of Sal B .MTT assay and PharmMapper sever were used to observe the cell via-bility and to screen the potential targets of Sal B , respectively .The predicted results were further evaluated by molecular docking program Autodock .Results Sal B at the concentrations of 100 μmol/L and 300 μmol/L significantly protected H9c2 cells from Dox-induced cell toxicity .The data from PharmMapper server and molecular docking analysis demon-strated that mitogen-activated protein kinase 12 ( MAPK12 ) and DNA topoisomerase 2 might be the potential targets of Sal B on the protective effect against Dox .Conclusion Sal B prevents H9c2 cells from Dox-induced H9c2 cell toxicity, and MAPK12 and DNA topoisomerase 2 may be involved in this action .
Keywords：salvianolic acid Bcardioprotectiondockingmitogen -activated protein kinase 12DNA topoi-somerase 2
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 3750-3753 )