Effects of salvigenin on humanγδT cell killing of colon cancer cell lines SW-620

CHEN Yang; FEI Su-juan; ZHOU Yu; LV Xiao-ting; LIU Jun-quan; CHEN Fu-xing; WU Ke-jian

Abstract：Objective To investigate the effects of salvigenin on human γδT cell killing of colon cancer cell lines SW-620.Methods Peripheral blood mononuclear cells were separated from healthy subjects and isopentenyl pyrophos -phate method was used to amplify γδT cells in vitro .AfterγδT cells were cultured with different concentrations of salvige-nin for 72 h, CCK8 assay was used to assess the proliferation of γδT cells.Lactate dehydrogenase release assay was ap-plied to measure the cytotoxic activity of γδT cells to colon cancer cells in vitro .Flow cytometry was performed to detect the expression of granzyme B (GraB), perforin (PFP) and CD107a of γδT cells after treatment.Western blot assay was used to test p-ERK1/2 expression in the treated γδT cells.Results After ten days of culture , the proportion of γδT cells increased from 4.32%to 83.63%.When salvigenin concentration was between 0.047 7 pmol/L and 195 pmol/L, the proliferation rate of γδT cells increased remarkably (P<0.05).When the concentration was between 0.191 pmol/L and 195 pmol/L, the cytotoxic activity of γδT cells to SW-620 cells enhanced significantly (P<0.05).After induction with salvigenin for 72 h, expressions of GraB, PFP and CD107a were significantly higher (P<0.05).When salvigenin concentration was between 0.763 pmol/L and 195 pmol/L, expression of p-ERK1/2 was significantly elevated .Conclu-sion At suitable concentrations , salvigenin can promote proliferation of human γδT cells and increase their cytotoxic ac-tivity against colon cancer cell SW -620.A possible mechanism may be up -regulated expressions of PFP and GraB and activation of the p-ERK1/2 signaling pathway .