Effects of a recombinant adenovirus vector expressing US3 gene on expression of interferon-γand perforin in ;mixed lymphocyte and NK reaction
WANG Peng
KAN Quan-cheng
LI Ling
PAN Xue
YU Zu-jiang
Abstract：Objective To investigate the activity of immune evasion induced by a recombinant adenovirus vector expressing the US3 gene in transfected peripheral blood mononuclear cells (PBMCs).Methods An adenovirus vector ex-pressing the US3 gene was established , multiplied and purified before being transfected into peripheral blood mononuclear cells ( PBMCs) .Immunological activities of US 3+cells were determined by testing the expression levels of interferon -γ( INF-γ) and perforin in mixed lymphocyte and NK cells reaction .Results r-US3 could significantly attenuate mixed lymphocyte and NK reaction by decreasing production of INF -γand perforin.Conclusion The adenovirus vector ex-pressing US3 gene may induce immune evasion in PBMCs with the transfected gene , attenuating rejection of the host im-mune system against transfected cells .
Keywords：immune evasionrecombinant adenovirusUS3interferon-γperforin
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 3105-3108 )