Effect of PEG10 on proliferation, invasion and migration of the A549 human lung cancer cell line

REN Ying-jie; GAO Yan-qiu; YANG Wei

Abstract：Objective To investigate the role of PEG10 in the proliferation, invasion and migration in A549 lung cancer cells, by implementing RNAi silencing of the PEG10 genein A549 cells.Methods A549 cells were cultured in RPMI-1640.Cell lines expressing PEG10-shRNAs were established through transient and stable transfection.Cell pro-liferation was assessed by MTT assay and soft agar colony formation assay for the effect of PEG10 silencing on proliferation of A549 cells.Scratch test and transwell assay were used to detect the cell migration and invasion.Expression of PEG10,β-catenin, cmyc, MMPs, twist, E-cadherin and Vimentin at both mRNA and protein levels were evaluated by reverse transcription PCR, real time PCR and Western blot.Results RNAi efficiency of PEG10 was 65%.Healing rate was slo-wer in the scratch test and quantity of viable cells in the transwell assay was lower after transfection, as well as colony forming units.Expression ofβ-catenin, c-myc, MMPs, twist and Vimentin were reduced while expression of E-cad-herin was elevated.Conclusion Blockage of PEG10 in A549 cells downregulates the expression ofβ-catenin, which is the critical factor of the Wnt/β-catenin signal pathway, inhibits proliferation, invasion and migration of the human lung cancer A549 cells, and partially reverses epithelial mesenchymal transition effect in lung cancer.