Effect of xenogeneic bone on induced differentiation of neural stem cells

QIU Shi-dong; WU Shi-fu; SUN Xiao-xia; ZHANG Lei; LI Na; HOU Li

Abstract：Objective To investigate the effect of xenogeneic bone on induced differentiation of neural stem cells (NSCs) .Methods NSCs were isolated from cerebral cortex of the frontal lobe in 15 d rat embryos and were cultured u-sing the neurosphere culture system.The basic culture medium was DMEM/F12,added with 2%B27,20μg/L EGF,20μg/L bFGF and others.After 3 passages,NSCs were stained by Nestin and identified by immunocytochemistry.They were then co-cultured with xenogeneic bone extracts at concentrations of 0.025,0.05,0.1,0.2 g/mL.Results Cells after passage exhibited characteristics of NSCs.Positive expression of microtubule-associated protein 2,glial fibrillary acidic protein and O4 were detected in NSCs co-cultured with 0.2 g/mL xenogeneic bone extracts.That is to say,xenogeneic bone extracts could induce NSCs differentiate into neurons,astrocytes and oligodendrocyte.When the concentration of xe-nogeneic bone extracts was 0.1 g/mL,the proportion of NSCs that differentiate into oligodendrocytes was the most.When the concentration was 0.05 g/mL,the proportion of NSCs that differentiate into astrocytes was the most.Conclusion Xe-nogeneic bone can induce NSCs to differentiate into neurons and glial cells.The proportion of differentiated cells was dif-ferent at various concentrations of xenogeneic bone.Therefore,in its clinical application,the xenogeneic bone combined with certain growth factors to induce NSC differentiation can help repair the damage of the nervous system.