Association of Fusobacterium nucleatum infection with colorectal cancer
LUO Yu-long
GE Quan-xing
NIE Yu-qiang
WANG Hong
DU Yan-lei
SHEN-bo
LI Yu-yuan
Abstract：Objective To investigate whether Fusobacterium nucleatum ( Fn) infection is associated with colorec-tal cancer ( CRC) .Methods The resected CRC ( pathologically confirmed) mass and matched normal samples from 101 patients were collected.Quantitative fluorescent polymerase chain reaction ( FQ-PCR) was applied to detect Fn in CRC versus matched normal tissues.Fluorescence in situ hybridization ( FISH) analysis was performed on 22 randomly selected CRC and matched samples to confirm FQ-PCR results.Abundance of Fn was analyzed for association with clinicopatho-logical parameters.Results Both analyses demonstrated that Fn was significantly over-represented in CRC.The rate of Fn over-abundance in CRC tissues versus that in matched normal tissues was 87.1%(88/101).The abundance of Fn was significantly greater in tumor tissues ( 0.242 ±0.034 ) than in matched normal tissues ( 0.050 ±0.017 ) ( P =0.000) .No significant association of Fn load with patients′gender, age, cancer location, stages and infiltration ( P>0.05) but with lymph node metastases (P=0.000) was observed.Conclusion Fn is enriched in the CRC tissues, which may be associated with CRC development and promote lymph node metastasis.
Keywords：Fusobacterium nucleatumcolorectal cancerFQ-PCRFISH
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2161-2164 )