Effects of umbilical cord mesenchymal stem cells transfected with interleukin-12 gene on gastric carcinoma cells
WANG Jiu-xiang
KANG Bai-hui
SI Ning-ning
ZHAO Fang-fei
LIU Xiao-zhi
Abstract：Objective To investigate the effects of umbilical cord mesenchymal stem cells ( UCMSCs) transfected with interleukin-12 (IL-12) gene on gastric carcinoma cells.Methods The isolated humanized UCMSCs were cul-tured and identified.UCMSCs were infected by the lentiviral vector ( LV-IL-12-GFP) harboring the IL-12 and green fluorescence protein ( GFP) genes.The expression level of IL-12 was measured by enzyme-linked immunosorbent as-says ( ELISA) .Gastric carcinoma cell line SGC-7901 cells were co-cultured with UCMSCs transfected with LV-IL-12-GFP.The invasion ability of SGC-7901 was monitored by the transwell dual chamber culture system, and the apop-tosis of SGC-7901 was detected by the in situ method.The migration ability of UCMSCs transfected with LV-IL-12-GFP towards SGC-7901 was monitored by Cylinder 48 hours later.Results UCMSCs were successfully isolated.The ELISA results showed the infected UCMSCs stably expressed a sustained high level of IL-12.The transwell results pre-sented the obviously reduced invasion ability and the increased apoptosis rate of SGC-7901 co-cultured with UCMSCs transfected with LV-IL-12-GFP (P<0.05).The Cylinder data revealed the obviously decreased ability of directional migration towards SGC-7901 of UCMSCs transfected with LV-IL-12-GFP ( P<0.05 ) .Conclusion UCMSCs can be used in the targeting therapy of gastric carcinoma cell as a vector of IL-12.
Keywords：gastric cancermesenchymal stem cellsgene therapyinterleukin-12
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1987-1990 )