The expression and significance of MAPK signal proteins in lung tissues of rats with acute lung injury induced by paraquat
LIU Zhen-ning
LIU Wei
GUO Feng
ZHAO Min
Abstract：Objective To investigate the expression of MAPK signal proteins in lung tissues of rats with acute lung injury induced by paraquat, and to evaluate its significance.Methods A total of 36 male SD rats were distributed to PQ and control groups equally and randomly.The subjects in PQ group were intraperitoneally injected with paraquat (20 mg/kg) and those in control group with saline.Peripheral blood and lung tissues were collected 8 hours, 1 and 3 days af-ter paraquat exposure.The level of MDA, the SOD activity, and the concentrations of TNF-α and IL-1βwere mea-sured via biochemical method and ELISA.Artery PaO2 was assessed with blood gas analyzer.The lung injury scores and the protein levels of MAPK pathway, including p-p38MAPK, p-JNK and p-ERK1/2, were also determined.Results Significant increase of TNF -α, IL -1βand MDA, and reduction in SOD activity in the peripheral blood were observedin rats of PQ group; so were the decline of artery PaO2 and elevation of lung injury scores in time -dependent manners.The levels of p -p38MAPK, p -JNK and p -ERK1/2 in lung tissues of rats in PQ group were also significantly increased. Conclusion Reactive oxygen species generated by paraquat can activate MAPK pathway, including p38MAPK,JNK and ERK1/2, leading to the acute lung injury.
Keywords：paraquatacute lung injuryp38MAPKJNKERK1/2
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1834-1837 )