Effects of glaucocalyxin A included by γ-cyclodextrin on S180 tumor growth and apoptosis

ZHANG Chong; QU Yan; SHANG Xiao-jun; BAI Su-ping

Abstract：Objective To investigate the effects of glaucocalyxin A included by γ-cyclodextrin ( GLA-γ-CD) on transplanted S180 tumor growth and apoptosis.Methods The transplanted S180 tumor models were established by implanting S180 cells into mice.The mice were randomly divided into 5 groups (n=10), control group (given with physio-logical saline), cyclophosphamide group (given with 10 mg/kg cyclophosphamide), GLA-γ-CD high, middle and low dose groups (given with 80, 40, and 20 mg/kg GLA-γ-CD, respectively).All drugs were given as intraperitoneal in-jection every day for 12 days.The effect of GLA-γ-CD on tumor growth was observed .Cell apoptosis was determined by TUNEL.The protein and mRNA expression of mutant p 53 was measured by immunohistochemistry and real -time fluo-rescence quantitative RT-PCR, respectively.Results Compared with control group , the tumor weight in each GLA -γ-CD group was significantly reduced (P<0.01, P<0.05).The tumor inhibition rate against S180 of GLA-γ-CD high dose group was 57.26%, and increased apoptosis index and down -regulation of mutant p 53 protein and mRNA (P<0.01) were observed, which were close to that of the cyclophosphamide group .Conclusion GLA-γ-CD can in-duce apoptosis and inhibit growth of tumor cells , which may be associated with regulating the expression of mutant p 53, with little cytotoxicity .