Effect of sesamin on angiotensinⅡ-induced cardiac fibroblast proliferation and collagen secretion

REN You-nan; ZHAO Meng-qiu; ZHENG Shu-guo; TAO Shan-jun; YANG Jie-ren; KONG Xiang

Abstract：Objective To investigate the effects of sesamin -containing serum on angiotensin Ⅱ( AngⅡ)-in-duced cardiac fibroblast ( CFB) proliferation and collagen synthesis , and the possible mechanisms .Methods CFBs were separated from neonatal rats with mixed enzyme digestion and purified by differential adhesion methods .After preincuba-tion with sesamin-containing rat serum for 12 hours, CFBs were exposed to Ang Ⅱ(10 -7 mol/L) for 24 hours.CFB pro-liferation was determined by MTT assay .Contents of collagen Ⅰ, collagen Ⅲ and TGF-β1 were determined by enzyme linked immunosorbent assay (ELISA).Levels of total antioxidant capacity (T-AOC) and malonaldehyde (MDA) were evaluated by colorimetric method .Phosphorylation of Smad3 was detected by Western blot analysis .Results Sesamin-containing serum effectively suppressed Ang Ⅱ-induced proliferation and collagen synthesis in CFBs , with significant re-duces in TGF-β1 content and Smad3 phosphorylation (P<0.05 or P<0.01).In addition, preincubation with sesamin -containing serum led to a significant increase of T -AOC and decrease of MDA content in CFBs .Preincubation with control serum had no significant influence on these observations mentioned above .Conclusion Sesamin-containing serum is capable of suppressing Ang Ⅱ-induced CFB proliferation and collagen synthesis , of which the underlying mechanisms might be related to attenuating oxidative stress and regulating TGF -β1/Smad signaling pathway .