Preliminary analysis about the expression and function of nitric oxide synthase in colon cancer
ZHONG Zhuo
WU Zhao-hui
DENG Hai-jun
LIU Zheng-jun
LIU Hao
ZHANG Qian-bing
ZHU Lin-qun
HUANG Wen-hua
LIU Qiu-zhen
Abstract：Objective To analyze different expression of the three kinds of NOSs , and thus to evaluate its role in colon cancer.Methods Flow cytometry, Western Blot , immunofluorescence and immunohistochemistry technology were used to analyze NOS expression in six colon cancer cell lines ( RKO, LOVO, SW480, SW620, HT29 and M5) and 10 tissue samples.NOS inhibitors were given for cell functional tests .Results NOS were mainly expressed in cytoplasm , while nucleus expression could be detected in certain condition .According to flow cytometry and Western blot , nNOS dis-played the highest expression (239 ±4.7~693.3 ±38 and 0.263 ±0.05~0.696 ±0.098, respectively), iNOS the ex-pression (42.7 ±13.6~236 ±34.0 and 0.079 ±0.04~0.291 ±0.006, respectively) and eNOS the lowest expression (13 ±7.4~68 ±13.6 and 0.019 ±0.004~0.123 ±0.004, respectively).Proliferation and invasion functions could be significantly inhibited by nNOS special inhibitor N -PLA and the non-selective NOS inhibitor L-NAME (42.5%, P<0.05), but limitedly inhibited by iNOS special inhibitor 1 400 W (9.7%, P>0.05).Conclusion The nNOS might play an important role in the proliferation and invasion of colon cancer , selective inhibition of nNOS might be an effective treatment for colon cancer .
Keywords：nitric oxide synthaseneuronal nitric oxide synthasenitric oxidecolon cancer
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1475-1479 )