The effects of the endothelin receptor blockade on severe acute pancreatitis and its inflammatory reactions

HUANG Ying; TAN Chao-chao

Abstract：Objective To investigate the effects of endothelin receptor blockade on severe acute pancreatitis (SAP)and inflammatory responses,thus to provide experimental basis for clinical application of endothelin antagonists. Methods Sixty male SD rats were randomly divided into control group(n = 12),SAP group(n = 24)and BQ123 group (n = 24). Normal saline was injected into control group,while caerulein was injected into subjects in SAP group and BQ123 group to build SAP models. The endothelin receptor antagonist BQ123 was injected into BQ123 group 4 hours after modeling,while replaced normal saline was given to the other two groups instead. The pathological changes of pancrea and lung,the serum amylase,blood urea nitrogen,creatine kinase,alanine aminotransferase,serum endothelin,inflammatory cytokines TNF - α,IL - 6,IL - 1,IL - 10 and pancreatic NF - κB signaling factors(IκBα of phosphorylation expres-sion)were determined. Results Significant elevation in pancreas and lung pathology scores,serum amylase,blood urea nitrogen,creatine kinase,alanine aminotransferase levels,serum inflammatory cytokine TNF - α,IL - 6,IL - 1,IL - 10 levels,and phosphorylated pancreatic IκBα were observed in SAP group than those in control group(P < 0. 05);which were significantly alleviated by BQ123. Conclusion The blocking of endothelin receptor in acute pancreatitis helps to im-prove microcirculation,reduce the damage of the pancrea and the other organs,and reduce stimulation of the NF - κB sig-naling pathway and the inflammatory response,thus prevent development of the pancreatitis.