Effects of chronic psychological stress on Prdx1 and Prdx3 proteins on human ovarian cancer xenograft

GAO Jun; ZHU Hong; GAO Guo-lan

Abstract：Objective To investigate the role of psychological stress in carcinogenesis and progression of ovarian cancer.Methods Nude mice were randomly divided into stress and control groups (n=10).Ovarian carcinoma xeno-graft model was established using SKOV3 subcutaneously injection in both stress and control groups.Stress mouse models were constructed using the restraint stress method in stress group.The growth of xenograft was observed.The differentially expressed proteins between stress group and control group were located, identified, and verified by proteomics methods. Combined with preliminary experiments, the possible associations between typical different expression proteins and tumor growth were analyzed.Results The growth of xenograft in stress group was faster than that in control group, with signifi-cantly heavier tumor weight (1.717 ±0.571 g vs 1.083 ±0.286 g, P<0.05).There were hundreds of differentially ex-pressed proteins in xenograft between stress group and control group, as 20 protein spots were changed by over two times, among which Prdx1 and Prdx3 proteins were significantly up-regulated in stress group (P<0.05).Conclusion Chronic psychological stress could promote the progression of ovarian carcinoma activating NF-κB pathway via Prdx1, suggesting Prdx1 and Prdx3 as potential targets for the treatment of ovarian cancer.