The correlation between MMP-9 gene polymorphism and chronic heart failure in Henan Han population
Guo-tian
ZHANG Yin-ke
HUANG Yan-mei
LI Jia-qi
DONG Shuang-shuang
XIE Na
SHANG Wan-bing
Abstract：Objective To study the correlation of single nucleotide polymorphisms ( SNPs) at MMP-9 gene with chronic heart failure ( CHF) among Henan Han population .Methods Polymerase chain reaction -restriction fragment length polymorphism ( PCR-RFLP) was used to detect the gene polymorphisms of three SNPs sites , including -1562C>T (rs3918242), R279Q (rs17576) and P574R (rs2250889), on MMP-9 gene.Alleles and genotypes were obtained according numbers and size of PCR products restricted .The data and the association between CHF and 3 SNPs on MMP-9 gene were analyzed with case -control study using SHEsis software .Results There was no significant difference in al-lelic frequency of MMP-9 gene -1562C>T between healthy subjects and patients (P>0.05), and the risk of CHF in the patients carried allele T was 1.25 times higher than that in those carried allele C (OR=1.254).The frequency of AG genotype of MMP-9 R279Q in patients with CHF was significantly increased (P<0.05).There was no significant differ-ence in genotype or allelic frequency of MMP -9 P574R between healthy subjects and CHF patients .There were signifi-cant difference in ACT and GCT haplotypes between the case group and the control group (P<0.05).Compared with the control group, the OR value of ACT haplotype was more than 1 in case group.ACT haplotype increased the risk of CHF . Conclusion In Henan Han population , T allele of MMP-9-1562C>T, AG genotype of MMP -9 R279Q and ACT GCT haplotype might increase the risk of CHF , while the allele and genotypes of P 574R on MMP-9 gene have no associa-tion with CHF .
Keywords：matrix metalloproteinases -9chronic heart failuresingle nucleotide polymorphismspolymerase chain reaction-restriction fragment length polymorphism
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 853-856 )