Effects of lipoxin A4 pretreament on expression of Caspase -3, Bcl -2 and Bax following myocardial ischemia -reperfusion injury in diabetic mellitus rats
ZHONG Yu-ling
XIE Yu-bo
MA Li
Abstract：Objective To investigate the effects of lipoxin A4 pre -treatment on expression of Caspase -3, Bcl -2 and Bax following myocardial ischemia -reperfusion injury (MIRI) in diabetic mellitus rats.Methods Sixty male Spra-gue -Dawley rats were randomly divided into 6 groups (n =10 each), normal rats sham operation group (CS ), normal rats myocardial I /R group (CI/R ), normal rats lipoxin A4 group (CLX ), diabetic rats sham operation group (DS ), diabet-ic rats myocardial I /R group (DI/R ) and diabetic rats lipoxin A4 group (DLX ).Diabetic mellitus was induced by intraper -itoneal STZ injection.MIRI was induced by 30 min occlusion of left anterior descending branch of coronary artery followed with 4 hours reperfusion.Rats were then sacrificed and myocardial specimens were isolated .The myocardial pathological pattern was detected by HE staining.The expression of Caspase -3, Bcl -2 and Bax was assessed by semi -quantitative reverse transcription polymerase chain reaction (RT -PCR) and Western blot methods, as well as the Bcl -2/Bax ratio was also calculated.Results The myocardial damages in Group C LX and Group DLX were significantly reduced.Significant down -regulation of Caspase -3 and Bax, and increase of Bcl -2 and Bcl -2/Bax ratio were revealed in Group C LX and Group DLX while compared with Group C I/R and Group D I/R , respectively.Conclusion Lipoxin A4 pretreament down -regulates the expression of Caspase -3 and Bax, up -regulates Bcl -2 expression, and increases the Bcl -2 /Bax ratio.
Keywords：lipoxin A4myocardial ischemia -reperfusion injuryCaspase -3Bcl -2Bax
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 670-672,673 )