Isolation and culture of trophoblast cells from human placenta in first trimester with different methods
ZUO Yan-zhen
HU Ya-tao
LI Yu-hong
XU Qian
Abstract：Objective To study the methodology for purification of human first trimester trophoblast cells from placentas with different methods , thus to seek a simple method for purification of human first trimester trophoblast cells in vitro.Methods Target cells isolated from first trimester human placenta with trypsin and collagenase Ⅰ were incubated directly; purified by differential time attachment and digestion ; purified by lymphocyte separation medium ; and dissociated in trypsin /collagenase Ⅰ with purification by simplified percoll gradient centrifugation , respectively.Morphologic observa-tion was applied with inverted phase contrast microscope , with purity was assessed by HE staining and immunocytochemis -try.Results Cytokeratin -7 positive cells were more than 90% only with the last method, while cytokeratin -7 positive cells were less than 60% with the other methods.Conclusion First trimester human trophoblast cells can be obtained through whole first trimester human placenta digestion and followed by simplified percoll gradient centrifugation purification .
Keywords：first trimester trophoblastprimary cell cultureplacentalymphocyte separation mediumpercollcytokeratin -7
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 665-669 )