Effects and mechanism of anacardic acid on human prostate cancer LNCaP cell cycle
LIU Ning-ning
DONG Xiao-xian
HUA Xian-liang
LIAO Yu-ning
HUANG Hong-biao
Abstract：Objective To observe the effects and mechanism on cell cycle of anacardic acid in human prostate cancer LNCaP cells.Methods MTS was used to detect cell proliferation rate of LNCaP cells treated with different con -centraions of anacardic acid at different time spots .The cell cycle analysis was determined by flow cytometry , and the changes of cell cycle related CDK4, CDK6, cyclin D1 and P27 were examined by Western blot.Results (1) MTS: the cell proliferation was significantly inhibited by anacardic acid in time -and dose -dependent manners; (2) Flow cytome-try: after treated by anacardic acid, cells growth were inhibited and mainly blocked at G 0 /G1 phase.(3) Western blot:after treated by anacardic acid , CDK4, CDK6 and cyclin D1 were down -regulated while P27 up -regulated.Conclusion Anacardic acid can inhibit LNCaP cell proliferation , which is mediated by the up -regulation of cell cycle -related pro-tein P27, the down -regulation of CDK4, CDK6 and cyclin D1, and the G0 /G1 phase arrest.
Keywords：anacardic acidprostate cancercell cycle
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 657-659 )