The expression of SATB1 and BRMS1 in breast invasive ductal carcinoma and their correlations with lymphatic ;metastasis

ZHANG Ying; CHEN Li-juan; LIU Yong-yuan

Abstract：Objective To investigate the expression of SATB 1 and BRMS1 in the tissue of the primary breast in-vasive ductal carcinoma ( IDC) , axillary nodes metastases and the normal breast tissues adjacent to carcinoma ;thus to an-alyze the correlation between SATB1 and clinicopathologic parameters , and the correlation between SATB1 and BRMS1. Methods The mRNA and proteins levees of SATB1 and BRMS1 in 51 samples of IDC tissues,17 samples of axillary nodes metastases tissues and 32 samples of the normal breast tissues adjacent to carcinoma tissues were detected by quanti -tative reverse transcription-polymerase chain reaction ( qRT-PCR) and immunohistochemical SP .Results The positive rates of SATB1 in IDC tissues and axillary nodes metastases tissues were 76.47%and 82.35%, respectively;significantly higher than that (28.12%) in the normal breast tissues adjacent to carcinoma (P<0.05).The positive rates of BRMS1 protein in IDC tissues and axillary nodes metastases tissues were 25.49%and 23.53%, respectively; significantly lower than that (87.5%) in the normal breast tissues adjacent to carcinoma (P<0.05).The levels of SATB1 mRNA were sig-nificantly 3.88-fold and 6.06-fold higher in IDC tissues and axillary nodes metastases tissues , respectively , than that in the normal breast tissues adjacent to carcinoma (P<0.05).The levels of BRMS1 mRNA were significantly 0.36-fold and 0.35-fold lower in IDC tissues and axillary nodes metastases tissues , respectively , than that in the normal breast tis-sues adjacent to carcinoma ( P<0.05 ) .There was no significant difference found between the IDC tissues and axillary nodes metastases tissues ( P>0.05) .There were significant differences in SATB 1 protein and mRNA and BRMS1 mRNA between the group of IDC with lymph node metastasis and the group of IDC without lymph node metastasis ( P<0.05 ) . The expression of SATB1 were significantly correlated with axillary nodes metastases and clinical stage , but not with age , tumor size, pathological grade, ER, PR, Her-2, Ki-67 or menopausal state (P>0.05).Conclusion The altered ex-pression of SATB1 and BRMS1 in IDC is correlated with the pathogenesis and lymph node metastasis , suggesting potential value in predicting lymph node metastasis of IDC .