The preservation of isolated mouse hearts by desiccation using carbon monoxide

ZHANG Zhong; XIAO Ze-zhou; GUO Yi-long; ZHOU Peng-yu; ZHU Ping; ZHENG Shao-yi

Abstract：Objective To investigate the protective effect of desiccation using carbon monoxide on isolated mouse hearts, and to provide a new preservation method for heart transplantation .Methods The modified Heron's technique for heterotopic cervical cardiotransplantation was used .C57BL/6 male mice (n=100) were used in this research.The donor mice (n=60, 4-6 weeks age) were randomly divided into 3 groups, HTK group (n=20), CO group (n=20) and Na-ive group (n=20).The receptor mice (n=40, 8-10 weeks age) were randomly divided into 2 groups, HTK group and CO group.The donor hearts from HTK group were preserved in HTK cardioplegic solution at 4℃ for 24 hours, and then transplanted to receptors .The donor hearts from CO group were hung in a high -pressure tank ( PO2 =3 200 hPa, PCO=800 hPa) at 4℃for 24 hours, and then transplanted to receptors .Meanwhile, the hearts from Na?ve group received no preservation.The re beat number of the donor heart was tested .The HO-1, LDH and CPK in myocardial cell 24 hours after reperfusion of the donor heart were assessed .Results The re-beat heart counts in HTK and CO groups were 7 (35%) and 15 (75%), with significant difference between them (P<0.05).The HO-1 in CO group was significantly higher than that in Naive group and HTK group .The LDH and CPK in CO group were significantly lower than those in HTK group ( P<0.05 ) .Comparing with Naive group , pathological changes were obviously observed in CO and HTK groups, including edema and inflammatory cell invasion;however, the injury was more prominent in HTK group .Conclu-sion Comparing with the traditional HTK preservation , the method of preservation by desiccation of using carbon monox-ide has a better effect for mouse isolated hearts for a long time preservation .