Effect of dexamethasone on the 18 F-FDG uptake capacity of hepatic carcinoma cells

XIE Liang-jun; QIN Lu-ping; LI Jian-fang; ZENG Feng-wei; YUAN Hai-juan; LIN Zhu-ge; ZHANG Feng; CHENG Mu-hua

Abstract：Objective To enhance the 18 F-FDG uptake capacity of hepatic carcinoma cells .Methods Hepatic carcinoma cell HepG2 and normal hepatic cell L02 were incubated for 2 h, 4 h, 6 h, 8 h, 12 h and 24 h with the dexam-ethasone of 10-8 mol/L,10-7 mol/L,10-6 mol/L,10-5 mol/L or 10-4 mol/L.The control group was treated without dexa -methasone.After that, 1 μCi 18 F -FDG was added for incubation into each group for 1 h, and the radioactivity counts were assessed subsequently.Results Dexamethasone significantly inhibited uptake capacity of the 18 F -FDG in HepG2 with the concentrations of 10-8 mol /L and 10-7 mol /L; promoted it with concentrations of 10-5 mol/L and 10-4 mol/L;and had no effect on it with the concentration of 10-6 mol/L (P <0.05).Meanwhile, Dexamethasone significantly inhibi -ted uptake capacity of the 18 F -FDG in L02 with the concentrations of 10-8 mol/L, 10-7 mol/L and 10-6 mol/L; promo-ted it with concentrations of 10-4 mol /L; and had no effect on it with the concentration of 10-5 mol/L (P <0.05).The 18 F -FDG uptake capacity of HepG2 was significantly higher than L02, and the difference was significantly enlarged by pre -treatment by dexamethasone (P <0.05).Conclusion Dexamethasone has biphasic effect on the 18 F -FDG uptake capacity of hepatocytes.The appropriate concentration of dexamethasone can enlarge the 18 F -FDG uptake difference be-tween HepG2 and L02.