Genome-wide analysis of retinoid X receptor αin the lipid metabolism of mouse livers
ZHAN Qi
NIE Yu-qiang
LI Yu-yuan
Abstract：Objective To characterize the genome targets of hepatic retinoid X receptor α( RXRα) as well as the correlation between its downstream pathways and lipid metabolism .Methods Six mice were divided into treatment group and control group (n=3), which were treated by retinoic acid 150 mg/(kg· d) or equal amounts of saline solution for 7 days, respectively.Serum lipid was detected.Hepatic DNA fractions bound by RXRαwere immunoprecipitated and sequenced .The distribution of RXRαbindings was characterized .Real-time PCR was used to evaluate the expression of RXRαtarget genes .Results Serum cholesterol and triglyceride were reduced significantly in treatment group compared to control group(P<0.05).RXRαbindings and target genes in treatment group were increased compared to control group , among which the overlapped transcriptional start site were increased the most by 58%.Genes implicated in oxidation re-duction, lipid metabolism and lipid transportation were targeted by RXRα.Among the genes associated with lipid metabo-lism in treatment group, 48%(11 out of 23) were down-regulated whereas 22%(5 out of 23) were up-regulated sig-nificantly.Conclusion RXRαbinds extensively to the mouse liver genome .It regulates the hepatic lipid metabolism at the transcriptional level .
Keywords：retinoid X receptor αlipid metabolismnuclear receptorchromatin immunoprecipitation sequen-cing
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 178-181 )