The effects of bile acid on the growth of scar fibroblasts in human intrahepatic bile ducts
DENG Shi-kang
YUAN Jun
WANG Tao
ZOU Hao
ZHANG Xiao-wen
Abstract：Objective To investigate the effects of bile acid on the growth of scar fibroblasts in human intrahepat-ic bile ducts.Methods Primary scar fibroblasts from human intrahepatic bile ducts were co-culture with bile acid.Mor-phological changes were observed under microscope.The growth of scar fibroblast was tested by MTT method, cell count and wound-healing test.Results The primary scar fibroblasts of intrahepatic bile ducts were successfully cultured.De-termined by MTT, significant promotion on the vitality of scar fibroblasts was observed in CA and DCA in the concentra-tions of 0.5, 1.0, 2.5, 5 μmol/L (P<0.05), while the other bile acids had no effect on the growth of scar fibroblasts (P>0.05).However, in concentration of 10, 20, 50, 100, 250, 500 μmol/L, the free bile acids and conjugated bile acids significantly inhibited the growth of scar fibroblasts (P<0.05).The count and vitality of the scar fibroblasts with the CA plus DCA, CA alone or DCA alone were significantly higher than the fibroblasts with CA plus DCA, CA alone and DCA alone (P>0.05).Conclusion Cholic acid and deoxycholic acid play important role in the formation of scar in the intrahepatic bile ducts.
Keywords：bile duct scarfibroblastbile acid
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2821-2824 )