The effects of siRNA on XPD gene expression and apoptosis of HUVECs
YU Ju-mei
SUN Guo-fang
LI Ju-xiang
HONG Kui
CHENG Xiao-shu
Abstract：Objective To study the effects on XPD gene expression and apoptosis of human umbilical vein endo-thelial cells (HUVECs) of transfecting small interfering RNA of xeroderma pigmentosum D.Methods Three specific re-combinant plasmids of XPD were constructed and identified.The silencing effects of XPDs in HUVCEs were assessed after siRNA transfection, and the siRNA with the best silence efficiency was selected among these 3 plasmids.Results West-ern blot and RT-PCR results showed that, compared with the control group, the transfection of all three recombinant plasmids of XPD-siRNAs significantly inhibited the expressions of XPD (P<0.01).Among these siRNAs, XPD-siR-NA-2 showed the best silence efficiency ( P<0.01 ) .Flow cytometry showed that, compared with control group, the XPD-siRNA-2 significantly reduced HUVCE apoptosis rate (P<0.01).Western blot results showed that, compared with control group, transfection of XPD-siRNA-2 significantly down-regulated the expression of Bax ( P<0.01 ) and up-regulated the expression of Bcl-2.Conclusion These findings prove that the expressions of XPD in HUVCEs can be effectively inhibited by the transfection of XPD-siRNA.The siRNA with the best silencing efficiency can inhibit the apoptosis of HUVEC, and Bax and Bcl-2 may take part in this process.
Keywords：siRNAXPDHUVCEtransfection
Publication Date：2014-09-16
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2817-2820 )