Effects of miR-146a transfection on nuclear factor-κB expression of alveolar macrophage
NIE Cheng
LIU Fen
ZENG Zhen-guo
ZHAN Yi-an
DENG Wen-gang
QING Cheng
SHAO Qiang
DING Cheng-zhi
JIE Ke-min
QIAN Ke-jian
Abstract：Objective To explore the plausible mechanism that miR-146a modulates the inflammatory response in alveolar macrophage by comparing nuclear factor-κB ( NF-κB) expression between cytoplasm and nucleus.Methods Alveolar macrophages from rat (NR8383) were divided into two groups: Group Transfected (transfected with Pre -miRTM miR -146a precursors) and Group Control (transfected with CyTM3 -labeled Pre -miRTM negative control).After24 -hour transfection, both groups were cultured in complete medium with lipopolysaccharide charge for 6 hours.NF -κBp65 proteins from cytoplasm and nucleus were extracted and determined by Western blot.Results Compared to CroupControl, miR -146a expression of Group Transfected was significantly up -regulated (P <0.01), so was the Compared toCroup Control, the cytoplasm NF -κB p65 expression of Group Transfected was also significantly up -regulated (P <0.05), while the nuclear NF -κB p65 expression was significantly down -regulated (P <0.05).Conclusion Transfectionof miR -146a into alveolar macrophage inhibits the NF -κB′s nuclear translocation, suggesting a mechanism viawhich miR -146a alleviates the inflammatory response in alveolar macrophages.
Keywords：alveolar macrophagemiRNA-146anuclear factor-κBnuclear translocationlipopolysaccharide
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2814-2816 )