The inhibition of all -trans retinoic acid on amyloid β-peptide 42 -induced apoptosis of astrocytes
HAN Xing
SHI Lei
YAN Hua-cheng
ZENG Xiao-hui
ZHANG Ying
GUAN Hui
ZHAO Shu-jin
Abstract：Objective To investigate the inhibitive effects of all-trans retinoic acid( ATRA) on amyloid β-peptide 42 ( Aβ42) -induced apoptosis of astrocytes.Methods The MTT assay was applied for detection of cell surviv-al, while qRT-PCR and Western blot were applied for assessment of ApoE and BACE1 expression.Astrocytes were cul-tured with different concentrations of ATRA for induction of apoptosis, while DMEM/F12 medium alone was used as con-trol.Results The effects of the different concentrations of ATRA on protecting against the Aβ42-induced apoptosis of astrocytes were different, as ATRA in 0.1 and 1 μmol/L significantly inhibited the apoptosis induced by Aβ42 ( P<0.05).Compared with control group, the expression of ApoE was significantly up-regulated by ATRA in 0.1 and 1μmol/L (P<0.01), while there was no significant difference in the expression of BACE1.Conclusion ATRA can up-regulate the expression of ApoE, prohibit the astrocytes apoptosis induced by Aβ42.Low concentration ATRA have protective effect on astrocytes.
Keywords：all-trans retinoic acidastrocytesamyloidβ-peptide 42ApoE
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2649-2651 )