Effects of cold water immersion and restraint stress on transient receptor potential vanilloid subtype-1 mRNA expression in different tissues of rats and the activities of SOD and MDA in serum
QIE Wen-bin
TU Wei-feng
WEN Jun-lin
XU Shao-qun
ZHOU Hong-yan
Abstract：Objective To investigate the effects of cold water immersion and restraint stress ( WIRS) of different duration on transient receptor potential vanilloid subtype -1 ( TRPV1 ) mRNA expression in rat dorsal root ganglion (DRG) and stomach tissue, and the activities of super oxide dismutase (SOD) and malondialdehyde (MDA) in serum. Methods Forty male Wistar rats were randomly assigned into 4 groups, including normal control group (Group NC), and 2 h-, 4 h-and 6 h-WRIS groups (Group WIRS 2h, WIRS 4h and WIRS 6h).The model of acute gastric muco-sal lesion ( AGML) was established by the classic WIRS method , and the general extent of gastric mucosal injury was ob-served and assessed for gastric mucosal injury index .HE staining was applied for observation of the degree of gastric mucosa injury.The quantification of TRPV1 mRNA expression in DRG and gastric tissue was performed using quantitative real -time PCR, and the activities of SOD and MDA in serum were also detected .Results Gastric mucosal injury was observed in all WIRS groups, with significant higher ulcer index than Group NC (P<0.05).Compared with Group NC, the TR-PV1 mRNA expression in stomach tissue from WIRS groups was all significantly reduced (P<0.05), while TRPV1 mR-NA expression in the thoracic DRG was significantly reduced in Group WIRS 6h (P<0.05).The activity of SOD in ser-um increased and reached peak at WIRS 4 h.The activity of MDA increased with the extension of stress time , but presen-ted temporary drop at WIRS 4h.Conclusion TRPV1 involves in the pathogenesis of AGML .The activities of SOD and MDA in serum reflect the oxidative and anti -oxidative capacities under stressing , which are expected to play important roles in discovering and evaluation of stress ulcer .
Keywords：cold water immersion and restraint stressacute gastric mucosal lesiontransiant receptor potential vanilliod subtype-1super oxide dismutasemalondialdehyde
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 2506-2510 )