Ethanol activates chloride channels in pancreatic acinar cells
HUANG Wei-yuan
MENG Long
ZOU Li-li
LI Meng-jia
WU Jia-bao
LAI Zhou-yi
WANG Yuan
CHEN Li-xin
WANG Li-Wei
Abstract：Objective To investigate the effects of ethanol ( EtOH) on the chloride channels in pancreatic acinar cells.Methods Whole -cell chloride currents were detected using the patch clamp technique .Results A weak and stablebackground chloride current was recorded under isotonic conditions .Extracellular perfusion with EtOH by 500 μmol/Linduced a strong outward -rectified current.There was no obvious voltage -and time -dependent inactivation in the appliedvoltages.In addition, both extracellular applications of 47% hypertonic solution and the chloride channel blockerNPPB (100 μmol/L) inhibited the current induced by EtOH.Conclusion Ethanol can activate the chloride channels inpancreatic acinar cells, which is suggested inducing acute alcoholic pancreatitis.
Keywords：EthanolChloride channelsPatch-clamp techniquesAcute alcoholic pancreatitisPancreatic aci-nar cells
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2502-2505 )