Effects of gambogic acid on the growth and angiogenesis of subcutaneous tumor xenografts from human colon cancer cell SW480 in nude mice established
LIANG Wen-long
CAO Jie
YANG Ping
LI Wang-lin
LIAO Shu-wen
ZHANG Tong
CHEN Xi-wen
WANG Qiang
SUN Zheng
Abstract：Objective To investigate the inhibitory effects of gambogic acid on the growth , tumor angiogenesis and expression of vascular endothelial growth factor receptor 2 (VEGFR2) of subcutaneous tumor xenografts in nude mice established from human colon cancer cell SW 480.Methods A nude mice model of colon cancer was established by sub-cutaneous inoculation of human colon cancer cell line SW 480.The mice were treated with different concentrations of gam-bogic acid since the 15th day after transplantation , and were sacrificed on the 28th day.Tumor volume and weight were measured , and the inhibition rate was calculated .HE staining was performed to identify the tumor necrosis and microves-sel morphological changes .The total RNAs and proteins in tumor xenografts were extracted .The mRNA and protein ex-pression of VEGFR2 in tumor xenografts were analyzed by RT -PCR and immunohistochemistry , respectively .The mi-crovessel density ( MVD) in tumor xenografts was observed by immunohistochemistry .Results By the end of treatment , the tumor volume of control group , gambogic acid low -, medium-and high-dosage groups were 691 ±67.2, 481 ± 62.9, 377 ±37.0 and 190 ±67.9mm3, respectively; while the tumor weights were 0.681 ±0.072, 0.473 ±0.062, 0.377 ±0.044 and 0.192 ±0.068 g, respectively .Through analysis of variance , the tumor volumes and weights of gam-bogic acid low-, medium-and high-dosage groups were significantly smaller than control group (all P<0.05), with inhibition rates of 31%, 45%and 72%, respectively .Coagulation necrosis and neovascularization atresia in tumor were observed in gambogic acid treatment groups .Compared with control group , VEGFR2 mRNA and protein in gambogic acid treatment groups were significantly reduced in concentration -dependent manners (P<0.05).The MVD in control group was significantly higher than those in the gambogic acid treatment groups ( P<0.05 ) .Conclusion Gambogic acid can significantly inhibit the growth and angiogenesis of subcutaneous tumor xenografts in nude mice established from human co -lon cancer cell SW480.Its mechanism may be related to the inhibition of VEGFR 2 expression.
Keywords：colonic neoplasmgambogic acidSW480 cancer cell linetumor angiogenesisvascular endothelial growth factor receptor 2
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2498-2501 )