Significance of transforming growth factor-β1 and Krebs Von den lungen-6 in plasma and bronchoalveolar lav-age fluid in children with bronchopulmonary dysplasia premature

HUANG Yong; ZHENG Qing

Abstract：Objective To observe the levels of TGF -β1 and KL-6 in plasma of bronchopulmonary dysplasia ( BPD) children before mechanical ventilation , to research its correlation with BPD , and the significance of TGF -β1 and KL-6 in plasma and BALF in different mechanical ventilation periods .Methods Ninety premature infants were selected and divide into 3 groups:non-mechanical ventilation group (Group NMV, n=30), non-BPD mechanical ventilation group (Group MV, n=30), and BPD mechanical ventilation group (Group BPD, n=30).Meanwhile, 30 full-term in-fants were enrolled as control group (Group C).TCG-β1 and KL-6 in plasma and BALF were assessed using ELISA . Results Before mechanical ventilation , the plasma TGF-β1 level in BPD infants was significantly higher than those in Group MV, NMV and C (P<0.0), and was significantly negatively correlated with gestational age (r=-0.274, P<0.01);while there was no significant difference in KL -6 among the four groups (P>0.05).At initial mechanical ventila-tion (1 hour), the TGF-βin BALF of BPD infants (200.5 ±63.8 μg/L) was higher than that in Group MV (137.5 ± 59.1 μg/L) , but with no significant difference .As the ventilation prolonged , the TGF-β1 and KL-6 in plasma and BALF in mechanical ventilation infants was increased; and significant changes in TGF -β1 nd KL-6 were observed in Group BPD since the 48th hour (P<0.01);while significant changes in TGF -β1 in plasma and BALF were not observed until the 48th and 72nd hour, respectively in Group MV (P<0.05), neither was the KL-6 in plasma until the 72nd(P<0.05).In different ventilation periods , TGF-β1 in plasma and BALF of BDP infants were significantly higher than those in is higher than those in non -BDP infants (P<0.05).No significant elevation in KL -6 was observed in BDP infants until the 48th hour (P<0.05).The correlation regression equation of TGF -β1 in plasma and BALF was Y=0.772 3X+10.664, with significant correlation (r=0.853, P<0.01).The correlation regression equation of KL -6 in plasma and BALF was Y=0.986 3X-16.195, with significant correlation (r=0.937, P<0.01).Conclusion High TGF-β1 in BALF before ventilation and at initial ventilation period suggests pulmonary hypoplasia of BPD infants .Dynamic monitoring of TGF-β1 and KL-6 in plasma during mechanical ventilation in premature infants is helpful to monitor the development of ventilator-induced lung injury .The TGF-β1 or KL-6 in plasma is significantly correlated with that in BALF .Dy-namic observation of TGF-β1 and KL-6 in BALF should be applied in early diagnosis and treatment of BPD .