Expression of urotensinⅡand its G protein-coupled receptor-14 in the pancreas of type 2 diabetes mellitus rats

LIU Fu-zhen; GAO Rui; HUANG Gui-xin; HUANG Xiao-jun; PENG Lin-ping; LI Jian-jian

Abstract：Objective To observe the expression of urotensin Ⅱ( UⅡ) and its G protein-coupled receptor-14 (GPR14) in the pancreas of rats with type 2 diabetes (T2DM), and further to study the correlation of UⅡ-GPR14 sys-tem and the metabolism of glucose , adipose and insulin in T2DM rats.Methods A total of 28 male Sprague -Dawley rats were randomly divided into two groups according to random number table :normal control group ( Group NC) and dia-betes mellitus group (Group DM);in which normal and high -fat diets were fed to the subjects, respectively.At the end of 8th week, intraperitoneal injection of STZ by 25 mg/kg was given to Group DM, while sterile sodium citrate was given to Group NC as control.At the end of 10th week, OGTT was used for selection of model rats.At the end of the 4th, 8th and 14th week, UⅡand the relevant metabolic indicators of glucose , adipose and insulin were measured .At the end of the 14th week, all the rats were sacrificed for collection of pancreatic tissue and pathological observation .The expression of UⅡand GPR14 in pancreatic tissues were assessed by immunohistochemical stretavidin proxidase ( SP) method and real-time fluorescent quantitative reverse transcription -polymerase chain reaction ( RT-PCR).Results At the end of the 8th week, the UⅡ, FPG, FINS, TG and TC in Group DM were significantly higher than those in Group NC (P<0.001), while HDL-C and ISI were significantly lower in Group DM (P<0.05);however, FINS at the end of 14th week was low-er than that at the end of the 8th week.At the end of the 14th week, the expressions of UⅡand GPR14 in Group DM were significantly up-regulated ( P<0.001) .Pearson linear correlation analysis shown that pancreatic UⅡ and GPR14 were significantly positively correlated with FPG , TG and TC ( P<0.05 ) , and negatively correlated with ISI and HDL -C (P<0.05).Conclusion The high expression of UⅡand GPR14 in the pancreas of rats with T2DM is correlated to the pathogenesis of T2DM.