The correlation between pulmonary leak and serum angiopoietin-2 in septic rats

LAI Tian-shun; LIN Zhe-wan; GUO Zhen-hui; XIAO Bai-fang

Abstract：Objective To observe the changes of lung permeability and serum angiopoietin -2 ( Ang-2) in ce-cal ligation and puncture ( CLP) induced sepsis in rats , and thus to investigate the correlation between serum Ang -2 and lung permeability.Methods Eighteen SD rats were randomly divided into control group (n=6) and sepsis group (n=12).The rats in sepsis group were modeled by CLP .Each 6 rats in sepsis group were sacrificed 12 h and 24 h after CLP, for assessment of the histological changes of lung , lung wet to dry weight ratio (W/D), total protein in bronchoalveolar lavage ( BALF) and serum Ang-2 concentrations .Results Serum Ang-2 concentration of rats in sepsis group was high-er than that in control group 12 h after CLP.In the sepsis group, with the time increased after CLP, the histological chan-ges were strengthened , and the lung W/D, total protein and serum Ang -2 concentration were also increased .Further-more, there was significantly positive correlation between serum Ang -2 and either W/D or total protein in BALF ( r=0.627, r=0.755, both P<0.05).Conclusion The increased lung permeability in sepsis rats induced by CLP is re-lates to blood Ang-2, which indicates that intervention that blocking the Ang -2 or related targets downstream its path-ways were helped to attenuate the lung leak in septic patients .