The collection,isolation,identification and culture of respiratory syncytial virus
LI Hong
QIAN Li
QIAO Jian-ou
Abstract：Objective To obtain live respiratory syncytial virus(RSV)from infected patients. Methods RSV in pharyngeal swab specimens collected from patients,after centrifugation,were identified by RT - PCR. RSV in positive specimens were further isolated and cultured through Hep - 2 cells,and the virus titers detected. Results Six RSV strains isolated from 60 specimens,with the positive rate of 10% ,were successfully cultured and passaged. Viral titer was 106. 33/ 100 μL. Conclusion RSV is a common pathogen of acute respiratory infections of infants. Using swabs,Hep - 2 cells and RT - PCR methods,virulent RSVs are successfully collected,isolated,and preliminary identified and cultured.
Keywords：respiratory syncytial viruspharyngeal swab specimensHep - 2 cells
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1659-1661 )