Effects of E2F - 1 overexpression on the proliferation of human gastric cancer cell line SGC - 7901 and its mecha-nism

CAO Wen-long; WEI Wei-yuan; ZHANG Xiao-shi; LUO Wen; YAN Lin-hai; XIE Yu-bo; XIAO Qiang

Abstract：Objective To investigate the effect of E2F -1 overexpression on the proliferation,growth and cell cy-cle of human gastric cancer cell line SGC -7901 and the molecular mechanism. Methods Humans gastric cancer SGC -7901 cells were infected by E2F - 1 recombinant lentiviral particles(LV - E2F - 1 - GFP),as experimental group(LV -E2F - 1 - GFP group),while control lentiviral particles(LV - GFP)infected human gastric cancer SGC - 7901 cells,as a negative control group(LV - GFP group)and blank control group was cultured conventionally without any treatment. CCK - 8 were used to detect cell proliferation activity,while flow cytometry was used for assessment of cell cycle distribu-tion. Semi - quantitative reserve transcription - polymerase chain reaction(RT - PCR)and Western blot were applied for measurement of mRNA and protein expression of Skp2,Bax,Bcl - 2,CylinD1 and Survivin. Results Compared with LV - GFP group and blank control group,significant lower proliferation activity(P < 0. 05),increased G0 / G1 phase pro-portion(P < 0. 05),down - regulation of mRNA and protein of Skp2,Bcl - 2,CylinD1 and Survivin(P < 0. 05),up -regulation of Bax mRNA and protein(P < 0. 05),were revealed LV - E2F - 1 - GFP group. Furthermore,there was no significant difference between LV - GFP group and blank control group(P > 0. 05). Conclusion Lentivirus - mediated overexpression of E2F - 1 induces growth inhibition of cancer cells,and cell cycle arrest at G0 / G1 phase;probably via up - regulation of Bax and down - regulation of Skp2,Survivin,Bcl - 2 and CylinD1.