The induction of resveratrol on apoptosis of CD34 + CD38 - KG-1a leukemia cells

HU Liang-shan; YANG Hua-wen; LI Li-hua; ZHANG Zhi-hong; FANG Xiao-ling; CAO Dong-lin

Abstract：Objective To observe the apoptotic effect of resveratrol on CD34 + CD38 - KG - 1a leukemia cells and to investigate the preliminary mechanism. Methods The expression of CD34 and CD38 on the surface of acute myeloid leukemia(AML)KG - 1a cells was assessed by flow cytometry. The effects of resveratrol at various concentration(12. 5, 25. 0,50. 0,100 and 200 μmol/ L)on the proliferation of KG - 1a cells and peripheral mononuclear cells were analyzed by trypan blue staining. The concentration of resveratrol that could inhibit the proliferation of about 50% KG - 1a was se-lected for subsequent experimental interventions. The changes of apoptosis in KG - 1a cells induced by resveratrol were as-sayed by flow cytometry through the staining of AnnexinV - FITC/ PI. The changes of Bcl - 2 family members,including Bcl - 2,Bcl - xl,Bid and Bax,in KG - 1a cells induced by resveratrol were assayed by real - time RT - PCR. The chan-ges of caspase - 3 in KG - 1a cells induced by resveratrol were assayed by spectrophotometry. Results The CD34 +CD38 - KG - 1a cells accounted for(90. 23 ± 2. 57)% cell count. Resveratrol inhibited the proliferation of KG - 1a leu-kemia cells in a concentration - dependent manner,while resveratrol at 25. 0 μmol/ L could inhibit the proliferation of a-bout 50% KG - 1a leukemia cells without effect on the proliferation of peripheral mononuclear cells. Early apoptosis(An-nexinV - FITC + PI - )and late apoptosis(AnnexinV - FITC + PI + )were induced by resveratrol at 25. 0 μmol/ L in KG -1a cells. Down - regulation of Bcl - 2 and Bcl - xl,with up - regulation of Bid and Bax,and increase caspase - 3 activity in KG - 1a cells were induced by resveratrol at 25. 0 μmol/ L. Conclusion Resveratrol induces apoptosis in KG - 1a cells by regulating the Bcl - 2 family gene expression.